Mass Humanization
   
         
   
  This platform is a radical new process to get access to human antibodies originally generated in immunized animals. It allows the immunization by different strategies (proteins, peptides, cDNA, proprietary rabbit cell lines) and enables a batch humanization process yielding fully human antibodies in a much faster and safer process than before.

Despite progress in capturing the immune system’s naïve antibody repertoire and optimization capabilities in synthetic libraries, immunized animals are still an attractive source of high affinity antibodies. However, humanization of such antibodies still represent a high risk as one may loose affinity, specificity, expressibility and/or stability We can meet the desire of our industry and generate antibodies in animals and turn such antibodies into human versions rapidly.

Rabbits produce high-quality antibodies and show strong immune response even against challenging targets. However, capacities of humanizations are usually limited and valuable candidates are too often lost or compromised in the process of humanization. AbCheck’s Rabbit Mass Humanization technology provides a new solution for the humanization of entire rabbit immune repertoires in one work flow, which reaches higher degrees of “humanness” compared to classic CDR grafting approaches. Based on four specifically selected human frameworks containing designed diversity in the CDR1 and 2 regions of VH and VL, unique RMH libraries are created by transferring rabbit diversity to the CDR3-regions.

The technology, developed in partnership with Distributed Bio, is a radical new approach for multi-parameter engineering and optimization of monoclonal therapeutics. By analyzing the mutated repertoire of rabbits and humans, we were able to pre-compute and pre-encode the complete humanization landscape in a "mass humanization" technology, in which we humanize the in vivo immune response of a rabbit in a single experiment.

Using Mass Humanization in discovery projects, AbCheck has repeatedly succeeded in identifying highly specific binders to challenging targets.

 
   
   
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